Androgen receptor Serine 81 phosphorylation is coupled to AR-mediated transactivation and co-sustained by CDK1 and CDK9 in prostate cancer

We examine total AR verus pSer81-phosphorylated AR chromatin-binding profiling in DHT-treated LNCaP cells to address the molecular functions of pS81 on the chromatin, and demonstrate that S81 phosphorylation can be mediated by both CDK1 and CDK9 at basal and androgen-stimulated levels. Overall design: 2 total AR and 3 pSer81-AR samples based on DHT-treated LNCaP cells are subjected to ChIP-Seq analyses

本研究以双氢睾酮（Dihydrotestosterone，DHT）处理的LNCaP细胞为研究模型，对总雄激素受体（Androgen Receptor，AR）及丝氨酸81位点磷酸化雄激素受体（pSer81-phosphorylated AR）的染色质结合谱进行分析，以解析S81磷酸化修饰在染色质层面的分子功能；实验证实，在基础状态与雄激素刺激条件下，CDK1与CDK9均可介导S81位点的磷酸化修饰。实验总体设计：基于DHT处理的LNCaP细胞，共设置2组总AR样本与3组pSer81-AR样本，所有样本均进行染色质免疫共沉淀测序（ChIP-Seq）分析。