Gene-regulatory potential of 25-hydroxyvitamin D3 and D2

In this study, we compared the modulation of the transcriptome of human PBMCs by the vitamin D metabolites 25-hydroxyvitamin D3 (25(OH)D3), 25(OH)D2 and 1a,25-dihydroxyvitamin D3 (1,25(OH)2D3). Overall design: PBMCs of a healthy male donor were isolated within one hour after peripheral blood collection and treated for 4, 8 or 24 h either with 1,25(OH)2D3 (0.1, 1 and 10 nM), 25(OH)2D3 (100, 250, 500, 750 and 1000 nM), 25(OH)D2 (100, 250, 500, 750 and 1000 nM) or solvent (0.1% EtOH).

本研究对比了维生素D代谢物25-羟基维生素D3（25-hydroxyvitamin D3，缩写25(OH)D3）、25(OH)D2与1α,25-二羟基维生素D3（1α,25-dihydroxyvitamin D3，缩写1,25(OH)2D3）对人外周血单个核细胞（Peripheral Blood Mononuclear Cells，简称PBMCs）转录组的调控作用。整体实验设计如下：健康男性供者的外周血采集后1小时内分离得到外周血单个核细胞，分别用1,25(OH)2D3（浓度设置为0.1、1和10 nM）、25(OH)2D3（浓度设置为100、250、500、750及1000 nM）、25(OH)D2（浓度设置为100、250、500、750及1000 nM）或溶剂（0.1%乙醇）处理4、8或24小时。