Genome-wide RNA-expression analysis after p53 activation in hepatocellular carcinoma

In order to characterize the differentially expressed mRNAs after the p53 activation ,RNA-seq were used after the overexpression of p53 in HepG2 cells. Overall design: Four samples of HepG2 cells were subjected to RNA-seq in two biological replicates.The HepG2 cells were treated with 1µg/ml doxorubicin for 24 hours to induce the expression of p53. The experimental group(dox-treated HepG2:HepG2_24h_rep1 and HepG2_24h_rep2) and control group(untreated HepG2: HepG2_0h_rep1 and HepG2_0h_rep2) were subjected to RNA-seq to identify the p53-regulated mRNAs.

为了表征p53激活后的差异表达mRNA，本研究在HepG2细胞中过表达p53后采用RNA测序（RNA-seq）进行分析。整体实验设计：本研究共设置4个HepG2细胞样本，分为实验组与对照组，每组各设2个生物学重复并开展RNA-seq。研究人员使用1µg/ml阿霉素处理HepG2细胞24小时以诱导p53表达，实验组为经该处理的HepG2细胞（样本标记为HepG2_24h_rep1与HepG2_24h_rep2），对照组为未接受处理的HepG2细胞（样本标记为HepG2_0h_rep1与HepG2_0h_rep2）。本研究通过RNA-seq鉴定受p53调控的mRNA。