Relationship of IFN-α responses and viral RNA.

aRNA was extracted from VRPΔErns-infected (MOI 5 TCID50/cell, 24 h) SK-6 cells before co-culture.bRNA was extracted from re-sorted enriched pDC (>99% CD172a+) treated with mock or infected withVRPΔErns (MOI 5 TCID50/cell, 22 h).cRNA was extracted from re-sorted enriched pDC co-cultured with VRPΔErns-infected SK-6 cells.SK-6 cells or enriched pDC were infected with VRPΔErns at an MOI 5 TCID50/cell and IFN-α in supernatants and viral RNA quantified after 24 h. In the last row, SK-6 cells infected with VRPΔErns-infected for 24 h were co-cultured with enriched pDC for further 22 h before IFN-α and viral RNA quantification. The latter used CD172a-re-sorted enriched pDC to remove contaminating SK-6 cells.

a) 共培养前，从经VRPΔErns感染（感染复数（MOI, Multiplicity of Infection）为5 TCID₅₀/细胞，感染时长24 h）的SK-6细胞中提取aRNA。
b) 从经模拟感染处理（空白对照）或VRPΔErns感染（MOI为5 TCID₅₀/细胞，感染时长22 h）的重分选富集型浆细胞样树突状细胞（pDC, plasmacytoid dendritic cell，纯度>99% CD172a阳性）中提取aRNA。
c) 从与VRPΔErns感染的SK-6细胞共培养的重分选富集型pDC中提取aRNA。
将SK-6细胞或富集型pDC以5 TCID₅₀/细胞的感染复数感染VRPΔErns，于感染24 h后对上清液中的干扰素-α（IFN-α, Interferon-α）及病毒RNA进行定量检测。
在最后一组实验中，将感染VRPΔErns 24 h的SK-6细胞与富集型pDC共培养22 h后，再对IFN-α与病毒RNA进行定量检测；该实验通过CD172a标记重分选富集型pDC以去除混杂的SK-6细胞。