Lipidomic analysis of moss species Bryum pseudotriquetrum and Physcomitrium patens under cold stress

Data based for the publication of the paper. Short abstract 

As non-vascular plants, which lack lignin for protection, bryophytes support themselves in harsh environment by producing various chemicals. In response to cold stress, lipids play a crucial role in cell adaptation and energy storage. Specifically, bryophytes survive at low temperatures by producing very long-chain polyunsaturated fatty acids (vl-PUFAs). However, a systematic knowledge and comprehensive understanding of the cold acclimation of bryophytes is limited. To overcome this obstacle, we performed lipid profiling using ultra-high performance liquid chromatography-quadrupole time of flight mass spectrometry (UHPLC-QTOF-MS) of two moss species (Bryum pseudotriquetrum and Physcomitrium patens) cultivated at standard condition compared to those cultivated at cold stressed condition. The potential biomarkers were identified by multivariate statistical analysis in each species. In B. pseudotriquetrum, we found that the phospholipids and glycolipids increased significantly under cold stress, while storage lipids decreased. The accumulation of the lipids with high unsaturation degrees (i.e. at least one fatty acyl chain contains more than two double bonds) mostly appear in phospholipids and glycolipids. However, this trend cannot be observed in P. patens. This suggests that different moss species may undergo a different lipid metabolic pathway of cold adaptation. Our findings present a deeper understanding of how mosses are adapted to cold temperature and provide a basis for future studies.

本数据集为该论文发表配套支撑数据，以下为论文简短摘要：

苔藓植物作为缺乏木质素保护的非维管植物，需通过合成多种化学物质以在严苛环境中维持生存。在低温胁迫响应过程中，脂质在细胞适应与能量存储中发挥关键作用。具体而言，苔藓植物可通过合成超长链多不饱和脂肪酸（very long-chain polyunsaturated fatty acids，vl-PUFAs）在低温环境下存活。然而，当前学界对苔藓植物低温驯化的系统性认知与全面理解仍较为匮乏。

为突破这一研究瓶颈，本研究采用超高效液相色谱-四极杆飞行时间质谱（ultra-high performance liquid chromatography-quadrupole time of flight mass spectrometry，UHPLC-QTOF-MS）技术，对分别在标准培养条件与低温胁迫条件下培养的两种苔藓物种（Bryum pseudotriquetrum和Physcomitrium patens）开展脂质组学分析，并通过多变量统计分析分别在两个物种中鉴定出潜在的低温胁迫生物标志物。在短尖土生银藓（B. pseudotriquetrum）中，我们发现低温胁迫下磷脂与糖脂含量显著升高，而存储脂质含量则呈下降趋势；高不饱和度脂质（即至少一条脂肪酰基链含有两个以上双键）的积累主要集中于磷脂与糖脂组分中。但这一变化趋势在小立碗藓（P. patens）中并未出现。

这一结果表明，不同苔藓物种可能采用不同的脂质代谢通路以适应低温环境。本研究结果加深了学界对苔藓植物低温适应机制的认知，并为后续相关研究提供了理论基础。