Transcription inhibition in Aedes Albopictus C6/36 cells. Transcription inhibition in Aedes Albopictus C6/36 cells

To determine codon optimality in Aedes Albopictus C6/36 cells, we blocked transcription using three independent transcription inhibitors (5,6-Dichlorobenzimidazole 1-β-D-ribofuranoside (DRB), Flavopiridol and Triptolide) and measured the RNA level at 6 hours post treatment using RNA-seq. Overall design: RNA-seq was performed at 6 hours after mock treated (DMSO) or blocking transcription with Flavopiridol, Triptolide or DRB.

为确定白纹伊蚊（Aedes Albopictus）C6/36细胞中的密码子最优性，我们采用三种独立的转录抑制剂——5,6-二氯苯并咪唑-1-β-D-呋喃核糖苷（DRB）、黄酮哌啶醇（Flavopiridol）与雷公藤内酯（Triptolide）——阻断细胞转录，并于处理后6小时通过RNA测序（RNA-seq）检测RNA水平。整体实验设计：分别在经模拟处理（二甲基亚砜，DMSO），或使用黄酮哌啶醇、雷公藤内酯、DRB阻断转录后的6小时，开展RNA测序。