RNA Sequencing Analysis of Wild Type and Non-phosphorylatable CARM1 mutation knock-in HEL cells

CARM1 is essential for the development and maintenance of myeloid neoplasms. We found that non-phosphorylatable CARM1 mutation impairs cell cycle progression, induces apoptosis, and downregulates stemness in JAK2-mutant cell lines, suggesting that CARM1 phosphorylation is required for maximal proliferation of myeloid neoplasms. Gene expression profile of wild-type or non-phosphorylatable CARM1 mutation (Y149F or Y334F single, or Y149F/Y334F double mutation) knock-in HEL cells.All cell lines sequencing experiments were repeated in triplicate. Differential expression of each non-phosphorylatable CARM1 mutation expressing cell line was performed independently comparing to wild-type.

CARM1是髓系肿瘤发生与维持过程中的关键调控因子。本研究发现，非磷酸化型CARM1突变会阻滞细胞周期进程、诱导细胞凋亡，并下调JAK2突变细胞系的干细胞干性，表明CARM1磷酸化对于髓系肿瘤的最大化增殖必不可少。本数据集为野生型或非磷酸化型CARM1突变（Y149F或Y334F单点突变，或Y149F/Y334F双点突变）敲入的HEL细胞系的基因表达谱。所有细胞系的测序实验均进行了三次生物学重复。针对每种携带非磷酸化型CARM1突变的细胞系，均独立以野生型细胞系为对照开展差异表达分析。