Combined RNA-seq and RAT-seq mapping of long noncoding RNAs in pluripotent reprogramming. Combined RNA-seq and RAT-seq mapping of long noncoding RNAs in pluripotent reprogramming

RNA transcriptome sequencing (RNA-Seq) and RNA reverse transcription-associated trap sequencing (RAT-Seq) were usded to map long noncoding RNA (lncRNAs) in reprogramming of fibroblasts into pluripotency Overall design: Fibroblasts (FIB) and induced pluripotent stem cells (iPSCs) were collected in the process of reprogramming. RNA-Seq was used to identify lncRNAs that are differentially-expressed in reprogramming. RAT-Seq was utilized to generate the database to map the regulatory element network of lncRNA candidate Osclr8 (NONMMUT043505, Platr10).

本研究采用RNA转录组测序（RNA transcriptome sequencing，简称RNA-Seq）与RNA反转录关联捕获测序（RNA reverse transcription-associated trap sequencing，简称RAT-Seq），对成纤维细胞重编程为多能干细胞过程中的长链非编码RNA（long noncoding RNA，简称lncRNAs）进行定位分析。总体实验设计：在成纤维细胞重编程进程中收集成纤维细胞（FIB）与诱导多能干细胞（induced pluripotent stem cells，简称iPSCs）；借助RNA-Seq筛选重编程过程中差异表达的长链非编码RNA；利用RAT-Seq构建数据库，以绘制候选长链非编码RNA Osclr8（NONMMUT043505，Platr10）的调控元件网络。