Quantitative Analysis of Wild Type and CISH-/- induced pluripotent stem cell derived NK cells (iPSC-NK) Transcriptomes

We developed human CISH-knockout (CISH-/-) NK cells using an induced pluripotent stem cell-derived NK cell (iPSC-NK cell) platform. And compare transcriptomes of CISH-/- iPSC NK cells with wild type iPSC NK cells using RNA seq. RNA seq data suggested that genes involved in the JAK-STAT signaling pathway and lymphocyte activations were significantly activated in CISH-/- iPSC NK cells. mRNA profiles of wild type and CISH knockout iPSC NK cells were generated by deep sequencing, in dulpicate, using Illumina HiSeq 2000.

本研究利用诱导多能干细胞衍生NK细胞（induced pluripotent stem cell-derived NK cell）平台，构建了人源CISH基因纯合敲除（CISH-/-）NK细胞。随后通过RNA测序（RNA-seq）对比CISH-/- iPSC-NK细胞与野生型iPSC-NK细胞的转录组。RNA测序数据显示，JAK-STAT信号通路（JAK-STAT signaling pathway）及淋巴细胞活化相关基因在CISH-/- iPSC-NK细胞中显著激活。本研究采用Illumina HiSeq 2000平台进行双重复深度测序，获得了野生型与CISH基因敲除iPSC-NK细胞的mRNA表达谱。