Additional file 12 of Gene networks and transcriptional regulators associated with liver cancer development and progression

Additional file 12. Master regulator molecules of genes upregulated in GE2-hepatocellular carcinoma (HCC). Significantly up regulated genes in GE2-HCC (n=13) versus NT (n=17) samples with mean fold change (FC) ≥ 2 and false discovery rate (FDR) ≤ 0.05 were identified (Additional file 5) and used as an input gene list for bioinformatics analyses. Master regulator molecules in the signal transduction pathways, at a distance of up to 10 steps upstream of the dysregulated genes, were identified using the curated database of pathways and protein interactions TRANSPATH®.

附加文件12：GE2型肝细胞癌（hepatocellular carcinoma, HCC）中上调基因的主调控分子（master regulator molecules）。本研究鉴定出GE2-HCC组（样本量n=13）相较于NT组（样本量n=17）样本中显著上调的基因，筛选标准为平均倍数变化（fold change, FC）≥2且错误发现率（false discovery rate, FDR）≤0.05（详见附加文件5），并将该基因集作为输入基因列表用于生物信息学分析。本研究通过经人工整理的通路与蛋白质相互作用数据库TRANSPATH®，鉴定出信号转导通路中位于失调基因上游至多10个信号层级的主调控分子。