PANDA epigenetically regulates the trade-off between panicle number and grain size in rice [RNA-Seq]

Next generation sequencing reveal profiles in H3K27me3 and expression levels between WT and panda mutant, anti-Flag ChIP reveal the binding sites of PANDA protein Overall design: Examination of H3K27me3 and expression levels in WT and panda mutant and PANDA binding sites in WT

下一代测序（Next generation sequencing）揭示了野生型（WT）与panda突变体间的H3K27me3修饰谱及基因表达水平；抗Flag染色质免疫沉淀（ChIP, Chromatin Immunoprecipitation）实验鉴定出PANDA蛋白的结合位点。整体实验设计：检测野生型与panda突变体的H3K27me3修饰水平、基因表达量，以及野生型样本中PANDA蛋白的结合位点。