Electrochemical sensing of hormonal serotonin levels in Crayfish

The neurotransmitter serotonin (5-hydroxytryptamine, 5-HT) has been implicated as a key biomarker that regulates a range of neurobehavioral functions. 5-HT also plays a prominent role in modulating social behavior across species. Here, we report the successful differentiation of hormonal (circulatory) 5-HT concentrations in socially isolated and communal crayfish samples by a surface-modified carbon fiber microelectrode electrochemical sensor. Monitoring 5-HT concentrations across social conditions provided important insights into mechanisms linking isolation to neurohormones that guide behavior. Utilizing surface coatings and electrochemical etching, our sensor can detect endogenous, nanomolar concentrations of 5-HT in vitro from crayfish hemolymph (i.e., blood) collected from communally housed animals and those isolated for one or seven days. Our measurements revealed that crayfish responses to isolation varied by species: Procambarus crayfish isolated for seven days exhibited a major..., All statistical analyses were performed using IBM SPSS (IBM Corp.). Ipa data is represented as the mean of measurements from a sample (n=3). Samples where no 5-HT was detected (Ipa=0 µA) were excluded from statistical analysis. Statistical comparisons among social groups and species were completed using a one-wayanalysis of variance (ANOVA). Differences between species were analyzed using non-parametric Kruskal-Wallis tests, followed by Mann-Whitney U tests for pair-wise comparison. Single-variable cross-species groups were evaluated using Mann-Whitney U tests. Statistical significance was defined at p<0.05.
, # Data from: Electrochemical sensing of hormonal serotonin levels in Crayfish

Dataset DOI: [10.5061/dryad.r2280gbs6](https://doi.org/10.5061/dryad.r2280gbs6)

## Description of the data and file structure

The excel sheet consists of raw data including the 5-HT concentration, Ipa values, weight, sex, label, social condition, and feeding conditions. The excel sheet lists all the values compiled and sorted for easier access. The first three tabs of the excel sheet includes the statistical output and input of the dataset, including ANOVA test, Kruskal-Wallis, and Mann-Whitney U. 

Multiple files in this repository require SPSS to generate. The SPSS files contain the raw input data for each test as well and statistical outputs.  

### Files and variables

#### File: Data_IsolateCommunal.xlsx

**Description:** Sorted raw data sheet including every experimental variable described below.  

##### Variables

* Data sorted by social condition, weight (in grams), sex, Ipa (in µA), 5-HT concentra...,

神经递质5-羟色胺（serotonin，5-羟色胺，5-hydroxytryptamine，缩写5-HT）是调控多种神经行为功能的关键生物标志物，其在跨物种的社交行为调控中亦发挥着重要作用。本研究成功利用表面修饰碳纤维微电极电化学传感器，区分了社交隔离与群居环境下螯虾的循环系统5-HT浓度。监测不同社交条件下的5-HT浓度，为解析社交隔离与调控行为的神经激素之间的关联机制提供了重要见解。本传感器通过表面涂层与电化学刻蚀技术，可在体外检测螯虾血淋巴（即血液）中的纳摩尔级内源性5-HT浓度，血淋巴采集自群居饲养以及隔离1天或7天的个体。研究结果显示，螯虾对隔离的响应因物种而异：隔离7天的原螯虾属（Procambarus）个体表现出显著变化……所有统计分析均使用IBM SPSS（IBM公司）完成。数据以样本测量均值表示（n=3）。未检测到5-HT的样本（Ipa=0 µA）被排除在统计分析之外。社交组与物种间的统计比较采用单因素方差分析（ANOVA）完成；物种间差异则采用非参数克鲁斯卡尔-沃利斯（Kruskal-Wallis）检验，随后辅以曼-惠特尼U（Mann-Whitney U）检验进行组间两两比较；单变量跨物种组则采用曼-惠特尼U检验进行评估。统计显著性阈值设定为p<0.05。

# 数据来自：《螯虾激素血清素水平的电化学传感》
数据集DOI：[10.5061/dryad.r2280gbs6](https://doi.org/10.5061/dryad.r2280gbs6)

## 数据与文件结构说明
本Excel工作表包含原始数据，涵盖5-HT浓度、Ipa值、体重、性别、标签、社交条件及饲养条件等字段。表格内所有数据均经整理排序，便于查阅。Excel的前三个标签页包含本数据集的统计分析输入与输出结果，涵盖单因素方差分析、克鲁斯卡尔-沃利斯检验及曼-惠特尼U检验的相关内容。
本仓库内多个文件需借助SPSS软件生成，SPSS文件同时包含各测试的原始输入数据与统计分析输出结果。

### 文件与变量
#### 文件：Data_IsolateCommunal.xlsx
**描述：** 经整理的原始数据表，包含下文所述的全部实验变量。
##### 变量
* 数据按社交条件、体重（单位：克）、性别、Ipa（单位：µA）、5-HT浓度……排序。