Opisthorchis viverrini infection exacerbates kidney disease through gut dysbiosis and alteration of host proteome in nonalcoholic steatohepatitis hamsters

Raw data about microbial 16S rRNA analysis used for Illumina Miseq sequencing platform based on the V3–V4 region of the 16S rRNA gene in Opisthorchis viverrini infection exacerbates kidney disease in nonalcoholic steatohepatitis hamsters model. This model used to identified combination of high fat and high fructose diets with Opisthorchis viverrini infection induced gut change in hamster feces. Twenty animals were divided into 4 groups; normal diet fed and non-infected Ov (Normal); HFF diets fed (HFF); Ov infection (Ov) and the last group fed with a combination of HFF diets and infected with Ov (HFFOv). An elevated levels of Ruminococaceae, Lachospiraceae, Desulfovibrionaceae and Akkermansiaceae while decreased level of Eggerthellaceae were seen in a combination treatment. Our finding suggests that combination of HFF diet and Ov infection can promote kidney injury and changes in gut microbiome. This knowledge may be an effective strategy for reducing or slowing down the progression of CKD.

本数据集包含适配Illumina MiSeq测序平台的微生物16S核糖体RNA（16S rRNA）分析原始数据，该分析基于16S rRNA基因V3-V4可变区，聚焦麝猫后睾吸虫（Opisthorchis viverrini, Ov）感染加重非酒精性脂肪性肝炎仓鼠模型肾脏病变的相关研究。该动物模型用于探究高脂高果糖饮食联合麝猫后睾吸虫感染对仓鼠粪便肠道菌群组成的调控作用。实验将20只仓鼠分为4组：正常饮食未感染组（Normal）、高脂高果糖饮食组（HFF）、麝猫后睾吸虫感染组（Ov）以及高脂高果糖饮食联合感染组（HFFOv）。联合干预组可见瘤胃球菌科（Ruminococcaceae）、毛螺菌科（Lachnospiraceae）、脱硫弧菌科（Desulfovibrionaceae）与嗜黏蛋白阿克曼菌科（Akkermansiaceae）丰度升高，埃格特菌科（Eggerthellaceae）丰度降低。本研究结果显示，高脂高果糖饮食联合麝猫后睾吸虫感染可加重肾脏损伤并改变肠道菌群组成。上述研究结果可为延缓或阻滞慢性肾脏病（chronic kidney disease, CKD）的进展提供潜在有效干预策略。