Pseudomonas sp. CES Genome sequencing and assembly

The CES strain was isolated from soil at the University of Iowa Center for Biocatalysis and Bioprocessing using an enrichment procedure with caffeine as sole carbon and nitrogen source. Prior to isolation, coffee grounds had been amended to the soil for ten days. The soil was then collected and incubated in M9 minimum medium with .25 percent caffeine as described previously previously (Yu CL, Louie TM, Summers R, Kale Y, Gopishetty S, Subramanian M. J Bacteriol. 2009 Jul;191(14):4624-32. Epub 2009 May 15.) The strain was initially characterized as Pseudomonas sp. Strain CES (for Coffee Enriched Soil) based on 16S rRNA sequencing and FAME analysis. CES uses the N-demethylation pathway for caffeine breakdown.

CES菌株（CES strain）从爱荷华大学生物催化与生物加工中心的土壤中分离得到，采用以咖啡因作为唯一碳源与氮源的富集培养法。分离前，研究人员已将咖啡渣施加至该土壤并培养十天。随后收集该土壤，置于含0.25%咖啡因的M9基础培养基（M9 minimum medium）中培养，操作方法参照此前发表的研究（Yu CL、Louie TM、Summers R、Kale Y、Gopishetty S、Subramanian M. 《细菌学杂志》(J Bacteriol)，2009年7月；191(14)：4624-4632，2009年5月15日提前在线发表）。该菌株最初通过16S rRNA测序（16S rRNA sequencing）与脂肪酸甲酯（FAME，fatty acid methyl ester）分析，被鉴定为假单胞菌属（Pseudomonas sp.）CES菌株（全称Coffee Enriched Soil，即咖啡富集土壤）。CES菌株通过N-脱甲基化途径（N-demethylation pathway）完成咖啡因的分解代谢。