Parameter values estimated by the proposed Bayesian approach using the experimental SPR response data.

Three tests were carried out. In the first experiment, the interaction data between CAII and 4-CBS were studied on a SensiQ Pioneer biosensor under a partial mass-transport limited condition. The data were analyzed and the resulted kinetic parameters and active concentration. In the other two tests, two datasets were collected from the literature. Dataset #1 was from the work done by Castonguay et al.[32], in which BMP10 interacted with immobilized human EngECD-Fc in a Biacore 3000 system. Dataset #2 was from a different work done by Abdiche et al.[33], in which human CGRPα bound immobilized 4901 IgG in a Proteon XPR36 biosensor. The two datasets were then analyzed by the proposed approach to estimate the active concentration of analyte A0, rate constants ka and kd, and the dissociation constant KD. The returned parameters as well as the parameters estimated in the original reference papers were listed. The 95% Bayesian credible intervals for the estimation were also provided in the square brackets. The biosensor used to collect the experimental data was indicated in the parentheses.Parameter values estimated by the proposed Bayesian approach using the experimental SPR response data.

本研究共开展三项实验。第一项实验中，我们借助SensiQ Pioneer型生物传感器（SensiQ Pioneer biosensor），在部分传质受限条件下，探究了CAII与4-CBS之间的相互作用数据，并对该数据进行分析以获取相应的动力学参数与活性浓度。另外两项测试的数据均来自已发表文献：数据集1源自Castonguay等人[32]的研究，即在Biacore 3000系统中，BMP10与固定化人源EngECD-Fc发生相互作用；数据集2源自Abdiche等人[33]的另一项研究，即在Proteon XPR36型生物传感器（Proteon XPR36 biosensor）中，人源CGRPα与固定化4901 IgG结合。随后采用本文提出的贝叶斯方法对这两个数据集进行分析，以估算分析物A0的活性浓度、结合速率常数$k_a$、解离速率常数$k_d$以及解离常数$K_D$。将本次估算得到的参数与原始参考文献中报道的参数一并列出，同时在方括号中标注出本次估算的95%贝叶斯可信区间（95% Bayesian credible intervals）。实验数据采集所用的生物传感器型号会标注在括号内。本部分为采用本文提出的贝叶斯方法，基于表面等离子体共振（Surface Plasmon Resonance, SPR）响应实验数据估算得到的参数值。