Antisense Cryptic transcription in histone H3 K36A, K56R and K36AK56R mutant yeast strain compared to wild-type

Gene expression microarray experiments to determine the existence of intragenic (cryptic) transcripts in K36A, K56R and K36AK56R mutant yeast strain compared to wildtype. Two color experiment. Mutant/WT. Biological replicates=3. Hybridisation of same samples to strand-specific gene expression arrays.

本数据集通过基因表达微阵列实验（gene expression microarray experiment），旨在对比野生型（Wildtype）酵母菌株与K36A、K56R及K36AK56R突变型酵母菌株中基因内（隐蔽）转录本（intragenic (cryptic) transcripts）的存在情况。该实验采用双色实验（two-color experiment）设计，以突变型/野生型（Mutant/WT）为检测信号比例，设置3次生物学重复；将同一样本与链特异性基因表达微阵列（strand-specific gene expression arrays）进行杂交。