Dataset for: Partitioning of iron and plutonium in exopolymeric substances and intracellular biopolymers: a comparison study between the coccolithophore Emiliania huxleyi and the diatom Skeletonema costatum

This dataset contains laboratory incubation experiments conducted using the coccolithophore Emiliania huxleyi and the diatom Skeletonema costatum, in the presence of 59Fe and 238Pu as radiotracers, in order to differentiate Fe and Pu uptake by extracellular exopolymeric substances (EPS) and intracellular biopolymers. The dataset compared the Fe and Pu distributions in select organic compound classes including proteins, total carbohydrates (TCHO) and uronic acids (URA) produced by these two types of phytoplankton, and includes the percentage of 59Fe and 238Pu activity and amounts of organic components in terms of µM-C, as well as the ratio of proteins to total carbohydrates (TCHO) and the percentage of the uronic acid (URA) in the bulk total carbohydrates pool, in different biopolymer fractions of E. huxleyi cells. Protein abundance was measured through a modified Lowry protein assay, using bovine serum albumin (BSA) as the standard. URA concentrations were determined by the metahydroxyphenyl method using glucuronic acid as the standard. This dataset supports the publication: Santschi, P., Quigg, A., Schwehr, K., & Xu, C. (2019). Partitioning of iron and plutonium in exopolymeric substances and intracellular biopolymers: a comparison study between the coccolithophore Emiliania huxleyi and the diatom Skeletonema costatum. Biological and Chemical Oceanography Data Management Office. doi:10.1575/1912/bco-dmo.764480.1

本数据集涵盖以赫氏颗石藻（Emiliania huxleyi）和中肋骨条藻（Skeletonema costatum）为实验材料开展的实验室培养实验，实验以59Fe和238Pu作为放射性示踪剂，旨在区分胞外聚合物（extracellular exopolymeric substances, EPS）与细胞内生物聚合物对铁（Fe）和钚（Pu）的摄取过程。本数据集对比了这两类浮游植物所产生的选定有机化合物组分中的铁与钚分布，所涉有机类别包括蛋白质、总碳水化合物（total carbohydrates, TCHO）及糖醛酸（uronic acids, URA）；数据集同时收录了59Fe与238Pu的活度占比、以微摩尔碳（µM-C）为单位的有机组分含量，以及赫氏颗石藻不同生物聚合物组分中蛋白质与总碳水化合物（TCHO）的比值、总碳水化合物库内糖醛酸（URA）所占百分比。蛋白质丰度通过改良Lowry蛋白质测定法定量，以牛血清白蛋白（bovine serum albumin, BSA）作为标准品；糖醛酸浓度采用间羟基苯酚法测定，以葡萄糖醛酸作为标准品。本数据集支撑以下学术出版物：Santschi, P.、Quigg, A.、Schwehr, K.与Xu, C.于2019年发表的《胞外聚合物与细胞内生物聚合物中铁与钚的分配：赫氏颗石藻与中肋骨条藻的比较研究》，刊载于生物与化学海洋学数据管理办公室（BCO-DMO），DOI: 10.1575/1912/bco-dmo.764480.1