转录组分析揭示了外生菌根真菌定植对柳树苗镉耐受性的功能影响（手稿 ID：1570200）-data.xlsx

Ectomycorrhizal fungus （ECMF）可以通过改变金属积累和保护植物免受氧化损伤来提高植物对重金属毒性的耐受性。然而，ECMF 介导的解毒之镉尚不清楚。本研究旨在揭示 Cenococcum geophilum（CG） 和 Suillus luteus（SL） 对 Cd 毒性耐受性Salix psammophila 'Huangpi1'。结果2 月龄柳树苗的生长、光合作用、抗氧化系统和转录组对 自 ECMFs 定植黄桐（S. luteus显著提高了地上部分的生物量，而C. geophilum 显著提高增强的根系特性集成电路柳树苗 Cd胁迫 差异表达基因 （DEGs） 的数量之间的比较中观察到CG+Cd （ 的 添加 100 μM Cd和 NF+Cd 无 ECMF 接种跟 100 μM Cd C. geophilum 定植 activated 植物激素信号转导和碳水化合物代谢途径，而 S. luteus 增强d生代谢物的合成。该 研究机制提供了s分子视角 柳树苗s 下的 Cd stress ，并支持 ECMF 在植物修复之受 CD 污染的土壤。

Ectomycorrhizal fungi (ECMFs) can enhance plant tolerance to heavy metal toxicity by altering metal accumulation and protecting plants from oxidative damage. However, the cadmium (Cd) detoxification mechanism mediated by ECMFs remains unclear. This study aimed to investigate the effects of *Cenococcum geophilum* (CG) and *Suillus luteus* (SL) on Cd toxicity tolerance in *Salix psammophila* 'Huangpi1'. For 2-month-old willow seedlings, the growth, photosynthesis, antioxidant system and transcriptome responses to Cd stress were analyzed. The results showed that inoculation with *S. luteus* significantly increased aboveground biomass, while *C. geophilum* significantly enhanced root traits of willow seedlings under Cd stress. A comparison of differentially expressed genes (DEGs) between the CG+Cd group (100 μM Cd addition with CG inoculation) and the NF+Cd group (no ECMF inoculation with 100 μM Cd) was performed. Inoculation with *C. geophilum* activated plant hormone signal transduction and carbohydrate metabolism pathways, while *S. luteus* enhanced the synthesis of secondary metabolites. This study provides a molecular perspective into the Cd stress response of willow seedlings, and supports the application of ECMFs in the phytoremediation of Cd-contaminated soils.