RNA-Seq data from NRC2/3/4 KO N. benthamiana expressing activated NLRs without cell death due to absence of helper NLRs

The aim of the project is to study transcriptional reprogramming downstream of NLR activation (for Sensors, Singletons and Helpers) in the absence of cell death. In this experiment, we use 4-week old NRC2/3/4 NLR helper knock-out mutant N. benthamiana plants which lacks the cell death when activated NLRs are expressed. Leaves from these plants were infiltrated with Agrobacteria GV3101 carrying activated NLR constructs and total RNA was harvested 48 h post infiltration for Illumina RNA-seq sequencing.

本项目旨在探究无细胞死亡状态下，NLR（核苷酸结合富亮氨酸重复序列受体，Nucleotide-binding Leucine-rich Repeat Receptor）激活后下游的转录重编程过程（针对感受器类、单分型及辅助型NLR）。本实验采用4周龄的NRC2/3/4型NLR辅助因子敲除突变体本氏烟草（Nicotiana benthamiana），该植株在表达激活型NLR时不会发生细胞死亡。将携带激活型NLR表达载体的农杆菌（Agrobacterium）GV3101浸润接种至该植株的叶片，并于浸润后48小时收集总RNA，用于Illumina RNA-seq测序。