Estrogen receptor alpha (ESR1) gene amplification is frequent in breast cancer. Subsequent Supplementary Video Clip S1+S2

<strong>Estrogen receptor alpha (<em>ESR1</em>) gene amplification is frequent in breast cancer. Subsequent Supplementary Material.</strong> Frederik Holst &amp; Christian Ruiz The supplementary material refers to: Holst, F., Stahl, P.R., Ruiz, C., et al. Estrogen receptor alpha (<em>ESR1</em>) gene amplification s frequent in breast cancer. Nature Genetics 39, 655-660 (2007). Subsequent Supplementary Figures S1-S7 Illustration of the different Affymetrix 10K SNP array gene copy number data analysis methods and regarding segmented <em>ESR1</em> copy number profiles of 22 breast cancers. A photo documentation of ESR1 FISH analysis signal appearance (figures 5A-C + 7A-C) of tumors with <em>ESR1</em> amplification by SNP GeneChip analysis is added. DOI: http://dx.doi.org/10.6084/m9.figshare.1470035 Subsequent Supplementary Video Clips S1+S2 The video clips document FISH analysis as performed for results documented in regarding subsequent supplementary figures 5A-C + 7A-C. DOI: http://dx.doi.org/10.6084/m9.figshare.1471677 Subsequent Supplementary Optical Dataset S1 The optical dataset consists of 94 photographs documenting appearance of <em>ESR1</em> FISH analysis signals of 20 out of 22 tumors regarding SNP GeneChip analysis illustrated in subsequent supplementary figures S1-S3 and S4+S6. The 94 photographs are separated into 1-10 figures per tumor including up to three z-stacks per figure. DOI: http://dx.doi.org/10.6084/m9.figshare.1470130 <strong>Methods References</strong> Ruiz, C. Doctoral Thesis, Universität Basel (2006). Moelans, C.B., Holst, F., Hellwinkel, O., Simon, R. &amp; van Diest, P.J. <em>ESR1</em> Amplification in Breast Cancer by Optimized RNase FISH: Frequent but Low-Level and Heterogeneous. PLoS One 8, e84189 (2013). Holst, F. FISH Analysis of <em>ESR1</em> Amplification in Breast Cancer - A Video Illustration. DOI: http://dx.doi.org/10.6084/m9.figshare.1471765 (2015).

<strong>雌激素受体α（Estrogen receptor alpha, <em>ESR1</em>）基因扩增在乳腺癌中频发。后续详见补充材料。</strong> 弗雷德里克·霍尔斯特与克里斯蒂安·鲁伊斯
补充材料对应文献：霍尔斯特F、施塔尔P.R.、鲁伊斯C.等. 乳腺癌中雌激素受体α（Estrogen receptor alpha, <em>ESR1</em>）基因扩增频发. *Nature Genetics*, 2007, 39: 655-660.
后续补充图S1~S7：阐释了不同Affymetrix 10K单核苷酸多态性（Single Nucleotide Polymorphism, SNP）阵列基因拷贝数数据分析方法，并展示了22例乳腺癌的分段式<em>ESR1</em>基因拷贝数图谱。
补充包含经SNP基因芯片分析确认存在<em>ESR1</em>扩增的肿瘤的<em>ESR1</em>荧光原位杂交（Fluorescence In Situ Hybridization, FISH）信号成像照片文档（对应图5A-C与7A-C）。数字对象标识符：http://dx.doi.org/10.6084/m9.figshare.1470035
后续补充视频片段S1与S2：该视频片段记录了对应补充图5A-C与7A-C中所呈现结果的FISH分析实验操作过程。数字对象标识符：http://dx.doi.org/10.6084/m9.figshare.1471677
后续补充光学数据集S1：该光学数据集包含94张照片，记录了经SNP基因芯片分析、对应补充图S1~S3及S4+S6所展示的22例肿瘤中20例的<em>ESR1</em> FISH分析信号成像结果。这94张照片按肿瘤分组，每组包含1~10张图像，每张图像最多包含3层Z轴堆叠成像。数字对象标识符：http://dx.doi.org/10.6084/m9.figshare.1470130
<strong>方法学参考文献</strong>
1. 鲁伊斯C. 博士学位论文. 巴塞尔大学（2006）.
2. 莫兰斯C.B.、霍尔斯特F、赫尔温克尔O、西蒙R、范·迪斯特P.J. 采用优化RNase荧光原位杂交技术检测乳腺癌中的<em>ESR1</em>基因扩增：频发但呈低水平及异质性分布. *PLoS One*, 2013, 8: e84189.
3. 霍尔斯特F. 乳腺癌<em>ESR1</em>基因扩增的FISH分析：视频演示. 数字对象标识符：http://dx.doi.org/10.6084/m9.figshare.1471765（2015）.