Assembled contigs from rhizosphere metatranscriptome. tom metatranscriptome

RNA was extracted from the rhizopheres of three crop plants and unplanted soil. rRNA was removed using Ribo-Zero and any remaining rRNA reads removed using SortMeRNA. Remaining high quality reads were assembled using Trinity

研究人员从三种农作物的根际（rhizosphere）及未种植土壤中提取总RNA。首先采用Ribo-Zero去除核糖体RNA（rRNA），再通过SortMeRNA移除残留的核糖体RNA测序读段，最终利用Trinity软件对剩余的高质量测序读段进行序列组装。