Comparative expression analysis of the Arabidopsis strigolactone (SL)-receptor mutant, d14-1, and wild type (WT) plants (Col-0) under dehydration conditions.

To understand the role of SL-signaling components in water stress response, we have carried out comparative expression analysis of the SL-receptor d14-1 mutant and WT plants under dehydration and well-watered (control) conditions. Aligent’s whole Arabidopsis Gene Expression Microarray (G2519F-021169, V4, 4x44K) was used. Two-week-old WT and d14-1 mutant plants were transferred from GM plates to soil and grown for 10 additional day. The aerial parts of 24-d-old plants were detached and exposed to dehydration on KimTowel papers for 0 (well-watered, control), 2 and 4 h. All rosette leaves of independent 24-d-old plants were collected. Total RNA was prepared and used for the microarray hybridization. Four independent biological replicates were used for each plant sample.

为探究独脚金内酯（Strigolactone，SL）信号通路组分在水分胁迫响应中的功能，本研究针对脱水胁迫与正常浇水（对照）条件下的SL受体d14-1突变体与野生型（wild type，WT）植株开展了比较表达分析。本实验采用安捷伦（Agilent）全基因组拟南芥基因表达芯片（货号G2519F-021169，V4版本，4×44K规格）。将两周龄的野生型与d14-1突变体植株从GM培养基平板移栽至土壤，继续培养10天。剪下24日龄植株的地上部分，置于KimTowel纸巾上进行脱水处理，处理时长分别为0小时（正常浇水，即对照）、2小时与4小时。收集各独立样本24日龄植株的全部莲座叶，提取总RNA并用于芯片杂交实验。每个植株样本设置4次独立生物学重复。