Additional file 12: of Assessing characteristics of RNA amplification methods for single cell RNA sequencing

Normalized read counts and expectation for ERCC transcripts. A–B. ERCC transcripts are found along the x-axis, ordered by expected number of input molecules. Axis labels are in the format of “ERCC spike-in ID, expected number of input molecules”. Points indicate the normalized read count for one transcript in one sample. Horizontal gray lines and background gray boxes indicate the expected normalized read count and a 95% CI under a Poisson model of dilution. Wide red horizontal lines indicate mean normalized read counts across all ERCC transcripts with a common expected number of input molecule, and red boxes indicate mean ± 2 × s.e.m. (A) aRNA. (B) SmartSeq Plus. (XLS 964 kb)

ERCC转录本的标准化读段计数与预期值。A至B图中，ERCC转录本沿横轴排布，排序依据为预期输入分子数。坐标轴标签格式为「ERCC外参转录本ID，预期输入分子数」。散点代表单个样本中单条转录本的标准化读段计数。灰色水平线与背景灰色框分别代表稀释泊松模型下的预期标准化读段计数及95%置信区间(95% CI)。宽幅红色水平线代表具有相同预期输入分子数的所有ERCC转录本的标准化读段计数平均值，红色框则代表平均值±2倍标准误(s.e.m.)。(A) aRNA；(B) SmartSeq Plus。(XLS 964 kb)