Gene expression profiling in acute murine autoimmune hepatitis. Mus musculus

The etiology of autoimmune hepatitis is poorly understood but likely involves Th1 cells producing IFN-γ. BALB/c background TGF-β1-/- mice rapidly develop fulminant Th1-mediated autoimmune hepatitis. Our aims are to profile liver gene expression in TGF-β1-/- mice, to identify gene expression pathways dependent on IFN-γ as possible targets for rational therapy, and to test potential targets directly in vivo in mice. Keywords: Comparative analysis of gene expression in livers of WT, TGFB1 & IFN knockout mice Overall design: DNA microarray analyses were applied to liver RNA from TGF-β1-/- mice, TGF-β1-/- /IFN-γ-/- mice, and TGF-β1+/+ littermate controls. 3 mice from each group were analyzed in this study.

自身免疫性肝炎的病因尚不明确，其发病可能与分泌γ干扰素（IFN-γ）的Th1细胞相关。BALB/c遗传背景的转化生长因子β1（TGF-β1）纯合敲除小鼠会快速进展为暴发性Th1细胞介导的自身免疫性肝炎。本研究的目标为：解析TGF-β1敲除小鼠的肝脏基因表达谱，鉴定依赖于IFN-γ的基因表达通路以作为合理治疗的潜在靶点，并在小鼠体内直接验证这些潜在靶点。
关键词：野生型（Wild Type, WT）、TGFB1与干扰素敲除小鼠的肝脏基因表达比较分析
实验设计：本研究对TGF-β1-/-小鼠、TGF-β1-/-/IFN-γ-/-小鼠以及TGF-β1+/+同窝对照小鼠的肝脏RNA开展了DNA微阵列分析，每组各使用3只小鼠进行实验。