Cell and molecular transitions during efficient dedifferentiation: population timecourses

Transcriptomic analysis of dedifferentiation in Dictyostelium discoideum, with comparison to forward development Overall design: RNAseq timecourses sampling the dedifferentiation of 14h developed cells in two different nutrient conditions; liquid growth media (Media, 0 to 18 h) and Klebsiella bacteria (Bacteria, 0 to 24 h), compared with mock dedifferentiation in phosphate buffer (Buffer, 0 to 6 h), a reference sample of undifferentiated cells (Undifferentiated) and a timecourse of forward development (Development, 0 to 14 h). All timecourses performed in duplicate.盘基网柄菌（Dictyostelium discoideum）去分化过程的转录组学分析，并与正向发育过程进行对比。整体实验设计：对两种不同营养条件下发育14小时的细胞的去分化过程开展RNA测序时间序列采样，分别为液体培养基（Media）组（0至18小时）与克雷伯氏菌（Bacteria）组（0至24小时）；同时设置磷酸缓冲液中的模拟去分化对照组（Buffer组，0至6小时）、未分化细胞参考样本组（Undifferentiated），以及正向发育时间序列样本组（Development组，0至14小时）。所有时间序列实验均设置两次生物学重复。