Functional study on the regulation of growth and development of Flammulina filiformis by FIP-fve gene

Flammulina filiformis belongs Tricholomataceae, Agaricales, Agaricomycetes, and is rich in protein, vitamins, and minerals. It is considered to have multiple nutritional and medicinal values. FIP-fve is a small molecule functional protein purified from the fruiting bodies of F. filiformis, which belongs to the family of Fungal Immunomodulatory Protein(FIPs). Although FIP-fve has been widely studied and considered to have potential medicinal value for cancer treatment and immune regulation, its biological function on the F. filiformis itself is not clear. In this study, we jointly analyzed the phenotypic statistics and transcriptome sequencing of FIP-fve gene overexpression, FIP-fve gene silenced, and wild-type F. filiformis to elucidate the mechanisms by which the FIP-fve gene affects the growth and development of F. filiformis and its possible mechanisms.

毛柄金钱菌（Flammulina filiformis）隶属于伞菌纲（Agaricomycetes）、伞菌目（Agaricales）、口蘑科（Tricholomataceae），富含蛋白质、维生素与矿物质，兼具多种营养与药用价值。FIP-fve是从毛柄金钱菌子实体中纯化得到的小分子功能蛋白，隶属于真菌免疫调节蛋白（Fungal Immunomodulatory Protein，FIPs）家族。尽管FIP-fve已被广泛研究，并被认为在癌症治疗与免疫调控方面具备潜在药用价值，但其针对毛柄金钱菌自身的生物学功能仍未明确。本研究针对FIP-fve基因过表达菌株、FIP-fve基因沉默菌株以及野生型毛柄金钱菌的表型统计与转录组测序数据开展联合分析，旨在阐明FIP-fve基因影响毛柄金钱菌生长发育的作用机制及其潜在调控途径。