Germination and vigor of soybean genotypes seeds under saline stress

ABSTRACT Soybean stands out among the crops with the greatest application of transgenics, mainly for tolerance to herbicides, pests, and diseases; however, studies on salt stress in genetically modified plants are scarce. This study aimed to evaluate the tolerance of both traditional and genetically modified soybean genotypes to saline stress during the germination and seedling phases. Seeds of 13 soybean genotypes were selected (five traditional (BRS Carnaúba, BRS Pérola, BRS Tracajá, BRS Sambaíba, and FTR-4389) and eight transgenic (BRS Sambaíba RR, BRS-333-RR, BRS-9820- RR, PAS-13565-74-RR, PAS-11711-007-RR, BRS-918-IPRO, AS-3810-IPRO, and M-8210-IPRO)), subjected to four osmotic potentials (0.0, 0.1, -0.2, and -0.3 MPa). The seed quality was evaluated using the following variables: the germination percentage, germination speed index, shoot length, root length, dry mass accumulation, and salinity tolerance index. All variables were found to be affected by salt stress. However, the conventional genotypes, BRS Carnaúba, BRS Pérola, BRS Tracajá, and BRS Sambaíba, and the RR group PAS-13565-74-RR, and PAS-11711-007-RR were tolerant to salinity, whereas the genotypes FTR-4389 (conventional) and BRS Sambaíba RR (RR) were less tolerant to salt stress, and all genotypes in the IPRO group were moderately tolerant to salt stress.

【摘要】 大豆是转基因技术应用最为广泛的作物之一，主要用于获得除草剂、虫害及病害耐受性；然而，针对转基因（genetically modified）植物盐胁迫的相关研究仍较为稀缺。本研究旨在评估传统基因型与转基因大豆基因型在萌发期及幼苗期对盐胁迫的耐受能力。本研究选取13个大豆基因型的种子：其中5个为传统基因型（BRS Carnaúba、BRS Pérola、BRS Tracajá、BRS Sambaíba及FTR-4389），8个为转基因基因型（BRS Sambaíba RR、BRS-333-RR、BRS-9820-RR、PAS-13565-74-RR、PAS-11711-007-RR、BRS-918-IPRO、AS-3810-IPRO及M-8210-IPRO），并设置4个渗透势梯度（0.0、-0.1、-0.2及-0.3 MPa）。随后通过发芽率、发芽速度指数、芽长、根长、干物质积累量及盐胁迫耐受指数这六项指标对种子品质进行评估。研究结果显示，所有测试指标均受到盐胁迫的显著影响。其中，传统基因型BRS Carnaúba、BRS Pérola、BRS Tracajá与BRS Sambaíba，以及RR组的PAS-13565-74-RR、PAS-11711-007-RR对盐胁迫表现出较强耐受性；而传统基因型FTR-4389与转基因基因型BRS Sambaíba RR的盐胁迫耐受性较弱，IPRO组的所有基因型均表现出中等程度的盐胁迫耐受性。