Drop-Seq analysis of mixtures of human (HEK) and mouse (3T3) cells (1000/100 STAMPs) at 12.5 cells per microliter

A cell supsension containing an equal mix of HEK and 3T3 cells was used in Drop-Seq, a novel technology for high-throughput single cell mRNAseq. Cells were mixed at a final concentration of 12.5 cells per microliter in droplets. Overall design: An estimated 570 STAMPs were selected for amplification.

本实验采用HEK细胞与3T3细胞等比例混合制备的细胞悬液，用于液滴单细胞测序（Drop-Seq）——一种面向高通量单细胞mRNA测序的新兴技术。细胞在液滴中的终浓度设置为每微升12.5个细胞。整体实验设计：预计选取570个STAMPs进行扩增。