Ancient synteny links metabolism with erythroid development. Ancient synteny links metabolism with erythroid development

In order to examine the link between Nprl3 regulation of cellular metabolism and erythropoiesis, we performed RNA-seq and identified gene expression changes in Nprl3 +/- and Nprl3 -/- mouse fetal liver erythroblasts compared with wild type Nprl3 +/+ erythroblasts. Overall design: Poly(A)+ mRNA was analysed from four Nprl3 +/+ fetal livers (2 male, 2 female), two Nprl3 +/- fetal livers (2 male) and two Nprl3 -/- fetal livers (1 male, 1 female).

为探究Nprl3对细胞代谢的调控与红细胞生成之间的关联，我们开展了RNA测序（RNA-seq）实验，对比分析了Nprl3 +/-、Nprl3 -/- 小鼠胎肝成红细胞与野生型Nprl3 +/+ 成红细胞的基因表达变化。
整体实验设计：本研究对4份Nprl3 +/+ 胎肝样本（2份雄性、2份雌性）、2份Nprl3 +/- 胎肝样本（均为雄性）以及2份Nprl3 -/- 胎肝样本（1份雄性、1份雌性）中的聚腺苷酸化mRNA（Poly(A)+ mRNA）进行了测序分析。