RNA-seq expression profiling of stromal and epithelial cell subpopulations from human breast tissue

Human breast samples were obtained from eight women with pathologically normal breast tissue and from three women with BRCA2 mutations. Total RNA was extracted from sorted luminal progenitor (Lin?/CD29lo/CD24+), mature luminal (Lin?/CD29hi/CD24+), basal and stromal cell populations. Mammary cell subpopulations were sorted from breast tissue from each of 11 individuals, three carrying BRCA2 mutations and eight with normal breast tissue. For one normal individual, tissue samples were obtained from both left and right breasts. Epithelial populations (basal, LP and ML) were sorted from all samples and, in addition, stroma cells were sorted from 6 of the normal samples. All RNA sample were profiled by RNA-seq. ------------------------------------- Submitter states " am unable to upload the raw sequence data files because of privacy concerns with human samples."

本研究的人体乳腺样本采集自8名经病理证实乳腺组织正常的女性，以及3名携带BRCA2基因突变的女性。研究人员从分选得到的管腔祖细胞（luminal progenitor, Lin⁻/CD29lo/CD24+）、成熟管腔细胞（mature luminal, Lin⁻/CD29hi/CD24+）、基底细胞与基质细胞群体中提取总RNA。乳腺细胞亚群从11名个体的乳腺组织中分选获得，其中3名携带BRCA2突变，8名乳腺组织正常。其中1名正常个体的样本同时采集自左右两侧乳腺组织。所有样本均分选得到上皮细胞亚群（包括基底细胞、管腔祖细胞LP与成熟管腔细胞ML）；此外，从6份正常乳腺样本中分选得到基质细胞。所有RNA样本均通过RNA测序（RNA-seq）完成转录组表达谱分析。
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本数据集提交者备注：“因人类样本涉及隐私问题，无法上传原始测序数据文件。”