Me-DIP-chip data from osteosarcoma cell lines. Homo sapiens

Malignant cells show major disruptions in DNA methylation profiles which manifest as aberrant hypermethylation and hypomethylation of gene promoters as well as global genomic hypomethylation. To date, many genes with aberrant promoter hypermethylation have been identified in essentially all forms of cancer including cell cycle regulators, DNA repair genes, genes associated with apoptosis, hormonal regulation, detoxification, metastasis, angiogenesis, and many others We developed an effective high-resolution approach for mapping genome-wide, and cancer-specific DNA methylation profiles. We have used this approach to provide first genomic DNA methylation profiling in osteosarcoma cells Keywords: comparative profiling Overall design: We utilized this platform in combination with the methylated DNA immunoprecipitation (Me-DIP) to develop a comprehensive approach for detection of hypo- and hypermethylation changes at high resolution, and used it to detect such changes in human osteosarcoma cells in relation to the normal human osteoblasts.

恶性细胞的DNA甲基化（DNA methylation）谱存在显著紊乱，表现为基因启动子的异常高甲基化与低甲基化，以及全基因组范围的低甲基化。迄今为止，几乎所有癌症类型中均已鉴定出存在启动子异常高甲基化的基因，涵盖细胞周期调控基因、DNA修复基因、凋亡相关基因、激素调控相关基因、解毒相关基因、转移相关基因、血管生成相关基因等诸多类别。本研究开发了一种高效的高分辨率全基因组癌症特异性DNA甲基化谱绘制方法，并利用该方法首次对骨肉瘤细胞完成了全基因组DNA甲基化谱分析。
关键词：比较谱分析
实验设计：我们将该平台与甲基化DNA免疫沉淀（methylated DNA immunoprecipitation, Me-DIP）技术相结合，开发出一套高分辨率检测低甲基化与高甲基化变化的综合方法，并利用该方法分析了人骨肉瘤细胞相较于正常人成骨细胞的甲基化变化。