Arabidopsis thaliana Raw sequence reads. Arabidopsis thaliana

Five-day-old seedlings grown under white light were treated with or without low R/FR (0.8) or very low R/FR (0.2) for 1 hr, and whole seedlings were collected. RNA-sequencing were conducted using a BGISEQ500 platform. Differentially expressed genes screening by NOISeq method. We find that shade induction of auxin response genes expression by very low R/FR (0.2) significantly decreased than that by low R/FR (0.8) in wide type, and therefore reflect the correlation between auxin and shade-induced hypocotyl elongation. Moreover, with 1hr low R/FR (0.8) treatment, there is no significant difference of shade induction between the two genotype, but the shade inductions of auxin responsive genes expression is significant enhanced in phyA mutant than that in Col-0 after 1 hr very low R/FR (0.2) treatment. Taken together, these data indicated the enhanced auxin response in phyA-211 under very low shade condition.

将在白光条件下培养5天的幼苗分为未处理对照组、低红光/远红光比值（R/FR，0.8）处理组及极低R/FR（0.2）处理组，处理时长均为1小时，随后收集全株幼苗。采用BGISEQ500平台开展RNA测序（RNA-sequencing），并通过NOISeq方法筛选差异表达基因。研究发现，在野生型（wild type）植株中，极低R/FR（0.2）处理诱导生长素响应基因表达的遮光效应显著低于低R/FR（0.8）处理组，由此反映出生长素与遮光诱导下胚轴伸长之间的相关性。此外，经1小时低R/FR（0.8）处理后，两种基因型植株的遮光诱导效应无显著差异；但经1小时极低R/FR（0.2）处理后，光敏素A（phyA）突变体中生长素响应基因表达的遮光诱导效应显著高于哥伦比亚生态型（Col-0）。综上，本研究数据表明，在极低遮光条件下，phyA-211植株的生长素响应水平显著上调。