Data Sheet 4_Unveiling unique metabolomic and transcriptomic profiles in three Brassicaceae crops.csv

Brassica napus, Camelina sativa and field pennycress (Thlaspi arvense), represent one highly economically valuable crop and two emerging oilseed crops of the Brassicaceae family, respectively. As sessile organisms, these crops are continuously exposed to various stresses when grown in the field. Interestingly, the responses of these three crops to different environmental stimuli vary to a great extent, but there is very limited knowledge about the molecular basis of these differential responses. In this study, we employed untargeted metabolomics to compare the metabolic profile of these crops, and examined the potentially related genes through further integration with transcriptomic analysis. Our data revealed distinctive overall metabolic profiles among these three crops, where in particular, a variety of phenylpropanoids showed differential accumulation and the corresponding putative genes’ expression varied significantly. The results provide a valuable resource for those studying Brassicaceae species and will provide insight into the understanding of metabolic variation among these three important oilseed crops, and provide potential targets for the future breeding of stress tolerant crops.

甘蓝型油菜（Brassica napus）、亚麻荠（Camelina sativa）与遏蓝菜（Thlaspi arvense）分属十字花科（Brassicaceae），其中前者为该科极具经济价值的主栽油料作物，后两者则为该科的两种新兴油料作物。作为固着生长的生物，这三种作物在田间种植过程中会持续面临各类逆境胁迫。值得注意的是，这三种作物对不同环境刺激的响应差异显著，但目前对这类差异响应的分子机制所知甚少。本研究采用非靶向代谢组学（untargeted metabolomics）技术对这三种作物的代谢谱进行比较分析，并通过整合转录组学分析筛选潜在的关联基因。研究结果显示，三种作物的整体代谢谱存在显著差异，其中多种苯丙烷类物质呈现差异化积累，其对应的推定基因的表达水平亦存在显著变化。本研究结果可为十字花科物种的相关研究提供宝贵的数据资源，有助于深入理解这三种重要油料作物的代谢差异，并为未来耐胁迫作物的育种工作提供潜在靶点。