circRNA-sequencing of a lymph node in a primary effusion lymphoma patient

Total RNA was extracted from a lymph node of a primary effusion lymphoma patient and treated with Rnase R to degrade linear, but not circular, RNA followed by deep-sequencing (PE150). Overall design: A clinical lymph node sample was dissected from a patient with primary effusion lymphoma for RNA extraction, RNase R treatment, and RNA-Seq.

从一名原发性渗出性淋巴瘤（primary effusion lymphoma）患者的淋巴结中提取总RNA，经核糖核酸酶R（Rnase R）处理以降解线性RNA（而非环状RNA），随后开展深度测序（deep-sequencing，PE150）。整体实验设计：从一名原发性渗出性淋巴瘤患者体内获取临床淋巴结样本，用于RNA提取、核糖核酸酶R处理及RNA测序（RNA-Seq）。