Delayed administration of TAT.ARC protein attenuates focal ischemic brain injury and fosters recovery in the long-term: behavioral data of neuroscores and rota rod tests, daily temperature and body weight measures

Mice were subjected to 30 min MCAo or sham surgery and TAT.b-Gal or TAT.ARC were administered in the contralateral ventricle after 3h after the onset of ischemia and mice were observed over 28 days. DeSimoni Neuroscore was performed at indicated time points (Fig. 8) as described (De Simoni et al., 2003; Orsini et al., 2012) with some modifications. In brief, general health (Table 2) and specific focal assessments (Table 3) were separately scored, analyzed and finally added to form a summation score. Summative scores added up to a maximum of 43 points with more points meaning more deficits. Rota rod was assessed as described recently (Hoffmann et al., 2015) and the best run out of three replicates at a given time point was used for statistical analysis. Body temperature was measured daily as survival was documented. Body temperature was non-invasively assessed at the same time of the day prior to body weight measurements using subcutaneous transponders (IPTT-300, Bio Medic Data Systems), for unambiguous identification of mice in their home cages as described (Kort et al., 1998; Langer and Fietz, 2014).

小鼠接受30分钟大脑中动脉闭塞（Middle Cerebral Artery Occlusion, MCAO）或假手术处理，并在缺血发作后3小时于对侧脑室给予TAT.b-Gal或TAT.ARC，随后对小鼠进行为期28天的观察。于指定时间点（图8）进行DeSimoni神经功能评分，该方法参考De Simoni等（2003）及Orsini等（2012）的方案并稍作修改。简言之，将一般健康状况（表2）与特异性局灶性评估（表3）分别进行评分、分析，最终求和得到总评分。总评分最高为43分，评分越高提示神经功能缺损程度越重。转棒实验参考Hoffmann等（2015）近期的方案进行评估，于指定时间点取三次重复测试中的最优结果用于统计分析。每日测量小鼠体温并记录存活情况。体温采用无创方式检测：于每日固定时间、体重测量前，使用皮下植入式应答器（IPTT-300, Bio Medic Data Systems）进行检测，该方法可在饲养笼中精准识别小鼠，参考Kort等（1998）及Langer与Fietz（2014）的方案。