API mutant sequencing

The M. truncatula api mutant exhibits developmental (root hair) and symbiotic (altered infection by Sinorhizobium meliloti) phenotypes (Teillet et al 2008, Mol Plant Microbe Interact. 21:535-46. doi: 10.1094/MPMI-21-5-0535). Genetic analysis indicated that a single recessive mutation controlled the observed phenotype and located the mutation to a 500 kb interval on M. truncatula chromosome 4. To identify a candidate mutation in this interval controlling the api phenotype , the genome of an api mutant is sequenced by Illumina technology.蒺藜苜蓿（Medicago truncatula）的api突变体表现出发育（根毛）与共生（苜蓿中华根瘤菌（Sinorhizobium meliloti）侵染异常）相关表型（Teillet等，2008，《分子植物-微生物互作》（Mol Plant Microbe Interact.），21卷：535-546页，DOI: 10.1094/MPMI-21-5-0535）。遗传分析表明，单个隐性突变即可调控该观测表型，并将该突变位点定位至蒺藜苜蓿4号染色体上一个500 kb的基因组区间内。为鉴定该区间内调控api表型的候选突变位点，研究人员采用Illumina测序技术（Illumina technology）对api突变体的基因组进行了测序。