Global gene expression changes during immune complex-induced neutrophil activation (wild type and Card9‒/‒ cells). Mus musculus

--- Raw data of the Supplementary Table 1 of the Nature Communications article 'Neutrophil-specific deletion of the CARD9 gene expression regulator suppresses autoantibody-induced inflammation in vivo' We used microarray to obtain global gene expression data of immobilised immune complex-activated mouse neutrophils in the presence and absence of the gene expression regulator molecule CARD9. Overall design: Mouse neutrophils were isolated from the bone marrow and were plated on human serum albumin & anti-human serum albumin antibody-coated surfaces at a 4 x 106 cell concentration. Human serum albumin-coated surfaces served as controls. The cells were stimulated for 4 hours before RNA preparation. The experiments were done at 3 independent timepoints.

本数据集为发表于《自然-通讯》（Nature Communications）的学术论文《中性粒细胞特异性缺失CARD9基因表达调控因子可抑制体内自身抗体诱导的炎症》的补充表1原始数据。本研究通过微阵列（microarray）技术，获取了在基因表达调控分子CARD9存在与缺失的两种条件下，固定化免疫复合物激活的小鼠中性粒细胞的全基因组基因表达数据。实验设计概述：从骨髓中分离小鼠中性粒细胞，以4×10⁶个细胞的浓度接种于人血清白蛋白与抗人血清白蛋白抗体包被的培养表面，以仅包被人血清白蛋白的表面作为对照组。细胞在进行RNA提取前被刺激培养4小时，实验共设置3个独立重复时间点。