RNA sequncing of HCN3+ urinary tract pacemaker cells

Purpose: to analyze the mRNA content of highly purified HCN3+ urinary tract pacemaker cells compared to HCN3- neighboring cells Methods: we sorted HCN3+ and HCN3- cells from E18.5 WT embryos and analyzed their content using RNA-seq Results: Identified differentially expressed transcripts in HCN3+ cells compared to HCN3- cells Conclusions: our study presents the first whole transcriptome analysis of HCN3+ urinary tract pacemaker cells that would provide a basis for the characterization of the development and function of those cells RNA sequencing of HCN3+ urinary tract pacemaker cells in comparison to HCN3- cells研究目的：分析高度纯化的HCN3+尿路起搏细胞与HCN3-邻近细胞的信使核糖核酸（messenger RNA，mRNA）含量 实验方法：从胚胎发育18.5天（E18.5）的野生型（wild type，WT）胚胎中分选得到HCN3+与HCN3-细胞，并采用RNA测序（RNA-seq）技术对其转录本含量进行分析 实验结果：鉴定得到HCN3+细胞相较于HCN3-细胞的差异表达转录本 研究结论：本研究首次完成了HCN3+尿路起搏细胞的全转录组分析，可为该类细胞的发育与功能特征研究提供理论基础 HCN3+尿路起搏细胞与HCN3-细胞的RNA测序分析