Screening of differentially expressed genes in LSCC cells knocking down FSCN1. Screening of differentially expressed genes in LSCC cells knocking down FSCN1

Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumor with a poor prognosis. Fascin actin-bundling protein 1 (FSCN1) has been reported to play a crucial role in LSCC development and progression, but the underlying molecular mechanisms remain unknown. Here, a whole transcriptome microarray analysis was performed to screen for differentially expressed genes in FSCN1 knockdown cells. We identified 1063 differentially expressed mRNA transcripts. Functional annotation revealed that these differentially expressed genes (DEGs) were involved in multiple biological functions such as transcriptional regulation, response to radiation, focal adhesion, ECM-receptor interaction, steroid biosynthesis, and others. Through co-expression and protein-protein interaction analysis, we linked FSCN1 to novel functions, including defense response to virus and steroid biosynthesis. Overall design: Three independent siRNA transfections were performed to generate FSCN1 knockdown and control TU-177 cells, followed by total RNA extraction and integrity examination.

喉鳞状细胞癌（Laryngeal squamous cell carcinoma, LSCC）是一类常见恶性肿瘤，预后较差。肌动蛋白束蛋白1（Fascin actin-bundling protein 1, FSCN1）已被证实于LSCC的发生发展中发挥关键作用，但其具体分子机制仍未明确。本研究通过全转录组芯片分析，对FSCN1敲低细胞中的差异表达基因进行筛选，最终共鉴定得到1063个差异表达mRNA转录本。功能富集注释结果显示，这些差异表达基因（DEGs）涉及转录调控、辐射应答、黏着斑、细胞外基质-受体相互作用、类固醇生物合成等多种生物学功能。通过共表达分析与蛋白质-蛋白质相互作用分析，本研究将FSCN1与病毒防御应答、类固醇生物合成等全新生物学功能建立了关联。实验整体设计：通过三次独立的siRNA转染，分别构建FSCN1敲低组与对照组TU-177细胞，随后提取总RNA并对其完整性进行检测。