Host Factor Experiment (E_R_HTBE_H5N1)

Human tracheobronchial epithelial (HTBE) cells are considered to serve as a good correlate of influenza virus infection in the human respiratory tract. All HTBE cells used in this study were obtained from a single donor. mRNA-Seq analysis was used to profile the cellular transcriptome of HTBE cells at multiple time points in response to infection with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), and A/Vietnam/1203/04 (H5N1) HALo virus. The Influenza A/Vietnam/1203/04 (H5N1) HALo mutant virus is an attenuated H5N1 virus generated from wild-type Influenza A/Vietnam/1203/04 (H5N1) virus as described in Steel, J., et al. J Virol. 2009 Feb; 83(4):1742-53.

人类气管支气管上皮（Human tracheobronchial epithelial, HTBE）细胞被视作研究人类呼吸道流感病毒感染的可靠替代模型。本研究所用的全部HTBE细胞均来源于单一供体。本研究采用mRNA测序（mRNA-Seq）技术，对HTBE细胞在分别感染甲型流感病毒A/California/04/09（H1N1）、A/Wyoming/03/03（H3N2）以及A/Vietnam/1203/04（H5N1）HALo毒株后的多个时间点的细胞转录组进行了特征分析。其中A/Vietnam/1203/04（H5N1）HALo突变毒株是由野生型甲型流感A/Vietnam/1203/04（H5N1）毒株构建得到的减毒H5N1病毒，具体构建方法详见Steel J等人发表于《Journal of Virology》2009年2月，第83卷第4期，第1742-1753页的研究成果。