HNF1B induced three-dimensional genome analysis of patient-derived pancreas neoplasm organoids [ChIP-seq]

Three-dimensional (3D) genome structures are organized in a hierarchy of various module layers, including chromosome compartment, topologically associating domain, and enhancer-promoter loop. These modules are important in regulating cell type specific gene expression. Here, we aimed to elucidate how 3D genome organization is related to regulating subtype specific genes in pancreatic neoplasms. 3D genome structure analysis together with epigenomic and expression profiling using patient-derived organoids. HNF1B was overexpressed to identify its role in subtype specific genome modulation. **Please note that raw data are submitted to JGA (Japanese Genotype-phenotype Archive) with JGAS000514 accession number***

三维基因组（3D genome）结构以层级模块化体系排布，包含染色体区室（chromosome compartment）、拓扑关联结构域（topologically associating domain）及增强子-启动子环（enhancer-promoter loop）等多种模块化层级。此类模块化结构在调控细胞类型特异性基因表达中发挥关键作用。本研究旨在阐明三维基因组组织与胰腺肿瘤（pancreatic neoplasms）亚型特异性基因调控之间的内在关联。研究依托患者来源类器官（patient-derived organoids）开展三维基因组结构分析，并结合表观基因组谱与表达谱检测。本研究通过过表达HNF1B，以明确其在基因组亚型调控中的功能。**请注意，原始数据已提交至日本基因型-表型档案库（JGA, Japanese Genotype-phenotype Archive），登录编号为JGAS000514**