Generation of guard cell RNA-seq transcriptomes during progressive drought

The study aimed to generate guard cell-specific RNA-seq transcriptomes in Arabidopsis thaliana during progressive drought stress and rewatering, while optimizing the INTACT protocol for RNA preservation in leaf tissue. By comparing gene expression changes between guard cells and bulk leaf tissue, this research enhances our understanding of guard cell-specific responses to drought, identifies unique and shared expression patterns, and provides valuable transcriptomic data that can inform strategies for improving drought tolerance and water use efficiency in crops.

本研究旨在获取渐进式干旱胁迫及复水处理过程中，拟南芥（Arabidopsis thaliana）保卫细胞特异性的RNA-seq转录组，同时优化适用于叶片组织RNA保存的INTACT实验方案。通过对比保卫细胞与全叶片组织的基因表达差异，本研究加深了对保卫细胞特异性干旱响应机制的认知，鉴定出独特与共有的表达模式，并提供了极具价值的转录组数据，可为作物耐旱性与水分利用效率的改良策略提供理论参考。