Cryo-electron microscopy EPN/Grenoble BAG

ESRF is privileged to host two state-of-the-art microscopes. Our BAG unites the majority of local labs that utilise cryo-EM to address their diverse structural biology questions, including researchers from the EPN campus (IBS, ESRF, ILL and EMBL) and its vicinity (Cell & Plant Physiology Laboratory LPCV, Grenoble Institut des Neurosciences - GIN, Institute for Advanced Biosciences - IAB).
In a nutshell, approximately 30 principal investigators would greatly benefit from Krios time, with 100 different samples proposed. The high demand for EM beamtime is partially met by two Glacios microscopes available on the campus, currently charged with the pre-screening, but also frequently yielding satisfactory structural insights. Nonetheless, the use of a Krios is indispensable for challenging samples (with heterogeneity or limited concentration requiring a large number of images, small proteins, etc.). Only such “difficult cases” will be selected for Krios time at the BAG level.

欧洲同步辐射光源（ESRF）有幸配备两台顶尖级冷冻电镜（cryo-EM）。我们的BAG汇聚了绝大多数利用冷冻电镜解决各类结构生物学问题的本地实验室，成员涵盖来自EPN园区（IBS、ESRF、ILL与EMBL）及其周边区域的研究人员，包括细胞与植物生理学实验室LPCV、格勒诺布尔神经科学研究所GIN、高等生物科学研究所IAB等机构的研究者。
简言之，约30名首席研究员可通过Krios设备机时获得极大助力，目前已有100份不同样本提交使用申请。校园内配备的两台Glacios显微镜可部分满足电子显微镜束流机时的高需求，目前这些设备主要用于样本预筛查，且常能产出令人满意的结构解析结果。不过，针对具有异质性、浓度有限需大量成像、小型蛋白等特性的挑战性样本，使用Krios设备是不可或缺的。仅此类"疑难样本"将在BAG层面被遴选为Krios机时的使用对象。