Serotype-Cross-Reactive Immunoglobulin M Responses in Dengue Virus Infections Determined by Enzyme-Linked Immunosorbent Assay

We developed immunoglobulin M (IgM) antibody capture enzyme-linked immunosorbent assays (ELISAs) with four monovalent dengue virus antigens. We attempted to determine whether IgM responses in dengue virus infections are serotype specific or serotype cross-reactive. Serum samples from 14 confirmed dengue cases were examined. In these 14 cases, which consisted of 12 Japanese and 2 non-Japanese patients, infecting dengue virus serotypes were defined by reverse transcription-PCR. Thirteen of the 14 cases were IgM positive in ELISA. IgM responses were serotype cross-reactive in these 13 cases but were highest against infecting dengue virus serotype in 9 of the 13 cases. These results indicate that IgM responses are generally dengue serotype cross-reactive but that IgM levels are highest against the infecting serotype in most dengue cases.

本研究建立了搭载四种单价登革病毒抗原的免疫球蛋白M（IgM）抗体捕获酶联免疫吸附试验（ELISA）方法，旨在探究登革病毒感染后机体的IgM应答究竟为血清型特异性，还是呈现血清型交叉反应性。本研究对14例确诊登革热病例的血清样本进行了检测。该14例病例包含12名日本患者与2名非日本患者，其感染的登革病毒血清型均通过逆转录聚合酶链反应（reverse transcription-PCR）完成鉴定。14例病例中有13例在ELISA检测中呈IgM阳性。针对这13例病例的分析显示，其IgM应答均呈现血清型交叉反应性，但其中9例的IgM水平在针对自身感染的登革病毒血清型时达到峰值。本研究结果表明，登革病毒感染后的IgM应答整体呈现血清型交叉反应性，但在大多数病例中，IgM水平在针对自身感染的血清型时达到最高值。