The effect of EDI3 inhibition in MCF7 breast cancer cells. Homo sapiens

EDI3 was shown to be relevant in cell migration, adhesion and spreading. Gene expression analysis was performed to determine the effect of EDI3 silencing in MCF7 cells in order to gain insight into potential underlying mechanisms. Overall design: MCF7 cells were transfected with either scrambled or EDI3 siRNA. Three biological replicates each with three technical replicates were performed.

已有研究证实，EDI3与细胞迁移、细胞黏附及细胞铺展过程密切相关。为深入解析其潜在的作用机制，本研究通过基因表达分析，检测了EDI3沉默对MCF7细胞的影响。总体实验设计：将MCF7细胞分别转染乱序阴性对照小干扰RNA（scrambled siRNA）与EDI3靶向小干扰RNA（EDI3 siRNA）；实验共设置3次生物学重复，每一次生物学重复均包含3次技术重复。