Nanopore Metabarcoding of Tomato Root Endophytic Communities Under Humic Substances and Osmotic Stress Treatments

Nanopore amplicon sequencing data (16S-ITS-23S rRNA operon, ~4,500 bp) from tomato root endophytic communities grown in a sterile hydroponic system, exploring the effects of humic substances and osmotic stress. Samples were analyzed to assess shifts in seed-transmitted bacterial communities in response to humic substances, osmotic stress, and their combination. A PNA clamp was used to minimize host DNA amplification, enabling species-level bacterial profiling.

本数据集包含来自无菌水培系统中培育的番茄根系内生菌群的纳米孔扩增子测序（Nanopore amplicon sequencing）数据，测序靶标为16S-ITS-23S rRNA操纵子（16S-ITS-23S rRNA operon），序列长度约4500碱基对。本研究旨在探究腐殖质与渗透胁迫对菌群的影响，通过分析样本以评估种子传播的细菌群落在腐殖质、渗透胁迫及其联合处理下的群落变化。实验中采用肽核酸钳（PNA clamp）以最大限度减少宿主DNA扩增，从而实现细菌的物种水平群落表征。