Gene expression changes in the human diaphragm following cardiothoracic surgery

It is unknown how soon the diaphragm begins to start the process of atrophy following the start of MV. We hypothesized that genes responsible for maintaining diaphragmatic contractile function, stress response, energy transduction would be altered over the course of a 5 hour cardiothoracic surgery. Two diaphragm biopsies were obtained from 5 male patients (~67 +/- 11 years) undergoing cardiothoracic surgery. The first biopsy was obtained as soon as the diaphragm was exposed; the second biopsy was obtained as late in the surgery as possible (4.9 +/- 1.8 hours). We profiled mRNA from the 5 pairs of muscle biopsies with a microarray (Affymetrix Human Genome U133 Plus 2.0).

目前尚不明确，机械通气（Mechanical Ventilation，MV）启动后，膈肌会在多久后开始出现萎缩进程。本研究假设，在5小时心胸外科手术期间，负责维持膈肌收缩功能、应激反应及能量转导的相关基因的表达会发生改变。本研究纳入5名接受心胸外科手术的男性患者（年龄约67±11岁），为每名患者采集两份膈肌活检组织。第一份活检组织在膈肌刚暴露时即刻采集；第二份活检组织则在手术中尽可能晚的时点采集，两次采样的间隔时长为4.9±1.8小时。本研究采用基因芯片（Affymetrix Human Genome U133 Plus 2.0）对5组配对的肌肉活检组织的信使核糖核酸（mRNA）进行了表达谱分析。