PCR primer sequences designed for genes validated in this study.
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Samples of cDNA were diluted 1∶10, and 2.5 ul were used per PCR reaction for all genes except EHBP1, where 4 µl was used with 5x Brilliant III Ultra Fast SYBR Green QPCR Master Mix (Agilent). In all cases primer concentrations were optimised to 300 mM (S, sense strand; AS, antisense strand)
将互补DNA（complementary DNA，cDNA）样本按1:10比例稀释后，除EHBP1基因外，所有基因的聚合酶链式反应（Polymerase Chain Reaction，PCR）体系均取用2.5微升稀释后的样本；针对EHBP1基因，则取用4微升稀释样本，并配合5× Brilliant III超快速SYBR Green定量PCR预混液（Brilliant III Ultra Fast SYBR Green QPCR Master Mix，安捷伦（Agilent））。所有实验中，引物浓度均优化至300毫摩尔每升（S：正义链；AS：反义链）
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2015-12-02
