Genomic profiling of Brazilian gliomas. Homo sapiens

The aim of this study was to perform a genomic profiling of gliomas of Brazilian origin, using array-CGH, MSI analysis and to associate the genomic alterations with TERT and IDH1 mutation status, and correlate the molecular features with clinicopathological characteristics. Overall design: 65 frozen tissue specimen, including 7 pilocytic astrocytomas, 9 diffuse astrocytomas, 7 anaplastic astrocytomas and 42 glioblastomas, were submitted to 60K Agilent array-CGH platform in order to determine copy number aberrations (CNA)

本研究旨在通过阵列比较基因组杂交（array-CGH）、微卫星不稳定性（MSI）分析对巴西来源的神经胶质瘤进行基因组特征分析，并将基因组改变与TERT和IDH1突变状态相关联，同时将分子特征与临床病理特征进行关联。整体实验设计：共纳入65份冷冻组织标本，包括7例毛细胞型星形细胞瘤、9例弥漫型星形细胞瘤、7例间变性星形细胞瘤以及42例胶质母细胞瘤，采用60K安捷伦阵列比较基因组杂交平台进行检测，以确定拷贝数变异（CNA）。