Gene expression data from T47D human breast ductal carcinoma cells treated with prolactin and/or NIM811

The hormone prolactin is implicated in the pathogenesis of breast cancer, and a subset of prolactin-induced gene expression is mediated by CypA activity. The non-immunosuppressive prolyl isomerase inhibitor, NIM811, is an inhibitor of CypA. We used NIM811 in combination with prolactin treatment in T47D cells to determine differential expression under these conditions. T47D cells were serum starved for 24 hours prior to treatment with NIM811 and/or prolactin, and subsequent RNA extraction.

催乳素（prolactin）参与乳腺癌的发病机制，其诱导的部分基因表达过程由亲环蛋白A（CypA）的活性所介导。非免疫抑制性脯氨酰异构酶抑制剂NIM811是CypA的抑制剂。本研究将NIM811与催乳素联合处理T47D细胞，以明确该实验条件下的基因差异表达情况。实验前，T47D细胞先经血清饥饿处理24小时，随后分别以NIM811、催乳素或二者联合处理，并进行后续RNA提取。